tgf-β3 10 ng Search Results


10 ng/ml transforming growth factor-beta 3 (tgf-β3)  (Neuromics)
90
Neuromics 10 ng/ml transforming growth factor-beta 3 (tgf-β3)
In vitro cartilage engineering from bone marrow-derived mesenchymal stem cell (BMSC)-seeded porous scaffolds. BMSCs were isolated by plastic adherence from bone marrow aspirates and expanded in tissue culture flasks to passage 2 within defined expansion medium containing fetal bovine serum and fibroblast growth factor-2 under either normoxic (21% O 2 ) or hypoxic (3% O 2 ) incubator conditions. Thereafter, BMSCs were seeded at 10 million cells per cubic centimeter onto clinically approved, cylindrical, porous scaffolds composed of collagen or hyaluronic acid. BMSC-scaffold constructs were subsequently cultured under either normoxia or hypoxia for 14 days within serum-free chondrogenic medium containing transforming growth <t>factor-beta</t> 3 and dexamethasone.
10 Ng/Ml Transforming Growth Factor Beta 3 (Tgf β3), supplied by Neuromics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10 ng/ml transforming growth factor-beta 3 (tgf-β3)/product/Neuromics
Average 90 stars, based on 1 article reviews
10 ng/ml transforming growth factor-beta 3 (tgf-β3) - by Bioz Stars, 2026-02
90/100 stars
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10 ng/ml tgfβ3  (Signalway Antibody)
90
Signalway Antibody 10 ng/ml tgfβ3
In vitro cartilage engineering from bone marrow-derived mesenchymal stem cell (BMSC)-seeded porous scaffolds. BMSCs were isolated by plastic adherence from bone marrow aspirates and expanded in tissue culture flasks to passage 2 within defined expansion medium containing fetal bovine serum and fibroblast growth factor-2 under either normoxic (21% O 2 ) or hypoxic (3% O 2 ) incubator conditions. Thereafter, BMSCs were seeded at 10 million cells per cubic centimeter onto clinically approved, cylindrical, porous scaffolds composed of collagen or hyaluronic acid. BMSC-scaffold constructs were subsequently cultured under either normoxia or hypoxia for 14 days within serum-free chondrogenic medium containing transforming growth <t>factor-beta</t> 3 and dexamethasone.
10 Ng/Ml Tgfβ3, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10 ng/ml tgfβ3/product/Signalway Antibody
Average 90 stars, based on 1 article reviews
10 ng/ml tgfβ3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


In vitro cartilage engineering from bone marrow-derived mesenchymal stem cell (BMSC)-seeded porous scaffolds. BMSCs were isolated by plastic adherence from bone marrow aspirates and expanded in tissue culture flasks to passage 2 within defined expansion medium containing fetal bovine serum and fibroblast growth factor-2 under either normoxic (21% O 2 ) or hypoxic (3% O 2 ) incubator conditions. Thereafter, BMSCs were seeded at 10 million cells per cubic centimeter onto clinically approved, cylindrical, porous scaffolds composed of collagen or hyaluronic acid. BMSC-scaffold constructs were subsequently cultured under either normoxia or hypoxia for 14 days within serum-free chondrogenic medium containing transforming growth factor-beta 3 and dexamethasone.

Journal: Stem Cell Research & Therapy

Article Title: Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds

doi: 10.1186/s13287-015-0075-4

Figure Lengend Snippet: In vitro cartilage engineering from bone marrow-derived mesenchymal stem cell (BMSC)-seeded porous scaffolds. BMSCs were isolated by plastic adherence from bone marrow aspirates and expanded in tissue culture flasks to passage 2 within defined expansion medium containing fetal bovine serum and fibroblast growth factor-2 under either normoxic (21% O 2 ) or hypoxic (3% O 2 ) incubator conditions. Thereafter, BMSCs were seeded at 10 million cells per cubic centimeter onto clinically approved, cylindrical, porous scaffolds composed of collagen or hyaluronic acid. BMSC-scaffold constructs were subsequently cultured under either normoxia or hypoxia for 14 days within serum-free chondrogenic medium containing transforming growth factor-beta 3 and dexamethasone.

Article Snippet: BMSCs were suspended in chondrogenic medium consisting of DMEM containing 4.5 mg/mL D-glucose, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 100 mM HEPES, 100 U/mL penicillin, 100 μg/mL streptomycin, 0.29 mg/mL L-glutamine (all from Life Technologies) supplemented with 0.1 mM ascorbic acid 2-phosphate, 0.1 μM dexamethasone, 1x ITS + 1 premix (Sigma-Aldrich), and 10 ng/mL transforming growth factor-beta 3 (TGF-β3) (Neuromics Inc.).

Techniques: In Vitro, Derivative Assay, Isolation, Construct, Cell Culture

Expansion and trilineage differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). (A) Cell counts, (B) population doublings per day, and (C) cumulative population doublings at each passage during expansion of BMSCs under normoxia or hypoxia. Data points represent mean ± standard error of the mean of cells from six donors, and P values are listed. (D) Osteogenic differentiation of BMSCs verified with Alizarin Red S staining following expansion under hypoxia and monolayer culture within medium containing β-glycerophosphate, dexamethasone, and fetal bovine serum. (E) Adipogenic differentiation of BMSCs verified with Oil Red O staining following expansion under hypoxia and monolayer culture within medium containing isobutyl-1-methylxanthine (IBMX), indomethacin, dexamethasone, and fetal bovine serum. (F) Chondrogenic differentiation of BMSCs verified by safranin O staining following expansion under hypoxia and culture performed in pellets (left) or scaffolds composed of collagen (middle) or hyaluronic acid (right) submersed in a defined serum-free chondrogenic medium containing transforming growth factor-beta 3 and dexamethasone.

Journal: Stem Cell Research & Therapy

Article Title: Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds

doi: 10.1186/s13287-015-0075-4

Figure Lengend Snippet: Expansion and trilineage differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). (A) Cell counts, (B) population doublings per day, and (C) cumulative population doublings at each passage during expansion of BMSCs under normoxia or hypoxia. Data points represent mean ± standard error of the mean of cells from six donors, and P values are listed. (D) Osteogenic differentiation of BMSCs verified with Alizarin Red S staining following expansion under hypoxia and monolayer culture within medium containing β-glycerophosphate, dexamethasone, and fetal bovine serum. (E) Adipogenic differentiation of BMSCs verified with Oil Red O staining following expansion under hypoxia and monolayer culture within medium containing isobutyl-1-methylxanthine (IBMX), indomethacin, dexamethasone, and fetal bovine serum. (F) Chondrogenic differentiation of BMSCs verified by safranin O staining following expansion under hypoxia and culture performed in pellets (left) or scaffolds composed of collagen (middle) or hyaluronic acid (right) submersed in a defined serum-free chondrogenic medium containing transforming growth factor-beta 3 and dexamethasone.

Article Snippet: BMSCs were suspended in chondrogenic medium consisting of DMEM containing 4.5 mg/mL D-glucose, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 100 mM HEPES, 100 U/mL penicillin, 100 μg/mL streptomycin, 0.29 mg/mL L-glutamine (all from Life Technologies) supplemented with 0.1 mM ascorbic acid 2-phosphate, 0.1 μM dexamethasone, 1x ITS + 1 premix (Sigma-Aldrich), and 10 ng/mL transforming growth factor-beta 3 (TGF-β3) (Neuromics Inc.).

Techniques: Derivative Assay, Staining